초록접수 현황
| 20F-141 | 구연 발표 |
Flow cytometry analysis reveals differential immune cell population in control and aneurysmal human aortic tissue
Tae-Wook Noh1, Hyun-Chel Joo1, Seung-Jun Lee2, Young-Guk Ko2, In-Ho Seo3, Eui-Cheol Shin3
Department of Cardiovascular Surgery, Severance Cardiovascular Hospital, Yonsei University College of Medicine1,
Department of Cardiology, Severance Cardiovascular Hospital, Yonsei University College of Medicine2,
Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology3
Purpose : Abdominal aortic aneurysm (AAA), caused by progressive weakening and dilatation of the aortic wall, can lead to aortic rupture and life-threatening complications. To improve our understanding of AAA pathogenesis, we sought to identify the molecular alterations in each of the diverse aortic cell population using flow cytometry analysis.
Methods : We performed flow cytometry analyses of abdominal aorta aneurysm wall tissues. Cell extracted from human aortic tissue was analyzed and categorized by cluster identification using flow cytometry analysis. AAA related alterations were examined by comparing immune cell type proportion and gene expression profile between AAA and control sample
Results : We performed immune cell population analysis in 26 humans aortic tissue (20 AAA patients, 6 control). There was no significant differential population in NK cell, B cell, monocyte and dendritic cell between aortic tissue and peripheral blood in AAA patients. In CD8+ T cells analysis, senescent cell was significantly decreased in AAA tissue. Effector memory (T-EM) and effector memory cells re-expressing CD45RA (T-EMRA) were showed in differential population between aortic tissue and peripheral blood in AAA patients. In flow cytometry analysis comparing to control aorta, we identified altered γδ T cell population in aneurysmal tissue. Vδ2+ T cell population, one subtype of γδ T cell was significantly increased in aneurysmal tissue.
Conclusion : Our study revealed the immune cellular composition changes was in aneurysmal tissue and Vδ2+ T cell serves as a key new player in the pathogenesis of AAA.
Methods : We performed flow cytometry analyses of abdominal aorta aneurysm wall tissues. Cell extracted from human aortic tissue was analyzed and categorized by cluster identification using flow cytometry analysis. AAA related alterations were examined by comparing immune cell type proportion and gene expression profile between AAA and control sample
Results : We performed immune cell population analysis in 26 humans aortic tissue (20 AAA patients, 6 control). There was no significant differential population in NK cell, B cell, monocyte and dendritic cell between aortic tissue and peripheral blood in AAA patients. In CD8+ T cells analysis, senescent cell was significantly decreased in AAA tissue. Effector memory (T-EM) and effector memory cells re-expressing CD45RA (T-EMRA) were showed in differential population between aortic tissue and peripheral blood in AAA patients. In flow cytometry analysis comparing to control aorta, we identified altered γδ T cell population in aneurysmal tissue. Vδ2+ T cell population, one subtype of γδ T cell was significantly increased in aneurysmal tissue.
Conclusion : Our study revealed the immune cellular composition changes was in aneurysmal tissue and Vδ2+ T cell serves as a key new player in the pathogenesis of AAA.
첨부파일 : 2020 추계학회 초록 20200904.docx
책임저자: Hyun-Chel Joo
Department of Cardiovascular Surgery, Severance Cardiovascular Hospital, Yonsei University College of Medicine
발표자: Tae-Wook Noh, E-mail : heyookcs@gmail.com