초록접수 현황
| 20F-084 | 구연 발표 |
Intraoperative Fluorescent Image-guided Detection of Esophageal Cancer in Rabbit and Patient Specimens
Jiyun Rho¹,², Yu Hua Quan¹,², Byeong Hyeon Choi¹,², Kook Nam Han², Beop-Min Kim³, Young Ho Choi², Hyun Koo Kim¹,²
¹Department of Biomedical Sciences, College of Medicine, Korea University, Seoul, South Korea
²Department of Thoracic and Cardiovascular Surgery, Korea University Guro Hospital, College of Medicine, Korea University, Seoul, Korea
³Department of Interdisciplinary Bio/Micro Technology, College of Engineering, Korea University.
Purpose : The purpose of this study was to assess the possibility of intraoperative detection of esophageal cancer after intravenous injection of indocyanine green (ICG) in a preclinical and clinical model.
Methods : Forty-five rabbits were surgically implanted with a VX2 tumor into the esophageal muscular layer 2 weeks before esophagectomy. They received intravenous injection of ICG at different concentration and time. Twelve patients scheduled to undergo esophagectomy were also enrolled. All patients received 2 mg/kg of ICG intravenously at 3, 6, 12, or 24 hours before surgery. The fluorescent intensity was measured in all resected specimens from the rabbits and patients using a near-infrared (NIR) fluorescence imaging system after surgery.
Results : Esophageal tumors were successfully established in all rabbits, and fluorescent signals were detected in all animal and patient specimens. The fluorescent signal of the tumor-to-normal ratio (TNR) of rabbit esophagus was highest at 12 hours with 1 mg/kg (p=0.027), 2 mg/kg (p=0.01) and 5 mg/kg (p=0.002) of ICG. The TNR in patients was also highest at 12 hours (p=0.002), with only a 2 mg/kg ICG. None of the patients had major complications as injection of ICG.
Conclusion : NIR fluorescence imaging could visualize esophageal cancer after system injection of ICG. Studies of the optimizing injection methods through the pre-clinical and clinical trials confirmed that 2 mg/kg of ICG at 12 hours before surgery could be injected for the detection of tumor. However, since the clinical trials were conducted in a small number of patients, further studies are needed in a larger populations.
Methods : Forty-five rabbits were surgically implanted with a VX2 tumor into the esophageal muscular layer 2 weeks before esophagectomy. They received intravenous injection of ICG at different concentration and time. Twelve patients scheduled to undergo esophagectomy were also enrolled. All patients received 2 mg/kg of ICG intravenously at 3, 6, 12, or 24 hours before surgery. The fluorescent intensity was measured in all resected specimens from the rabbits and patients using a near-infrared (NIR) fluorescence imaging system after surgery.
Results : Esophageal tumors were successfully established in all rabbits, and fluorescent signals were detected in all animal and patient specimens. The fluorescent signal of the tumor-to-normal ratio (TNR) of rabbit esophagus was highest at 12 hours with 1 mg/kg (p=0.027), 2 mg/kg (p=0.01) and 5 mg/kg (p=0.002) of ICG. The TNR in patients was also highest at 12 hours (p=0.002), with only a 2 mg/kg ICG. None of the patients had major complications as injection of ICG.
Conclusion : NIR fluorescence imaging could visualize esophageal cancer after system injection of ICG. Studies of the optimizing injection methods through the pre-clinical and clinical trials confirmed that 2 mg/kg of ICG at 12 hours before surgery could be injected for the detection of tumor. However, since the clinical trials were conducted in a small number of patients, further studies are needed in a larger populations.
첨부파일 : figure.pptx
책임저자: Hyun Koo Kim
Department of Thoracic and Cardiovascular Surgery, Korea University Guro Hospital, College of Medicine, Korea University, Seoul, Korea
발표자: Jiyun Rho, E-mail : jiyun.r1219@gmail.com