초록접수 현황
| 19F-114 | 포스터 발표 |
The Simulated Microgravity affected on Proliferation and Migration Differently between Adenocarcinoma and Squamous Cell Carcinoma of the Lung
Kuk Hui Son³, Chi Bum Ahn¹, Ji-Hyun Lee¹, Sung-Ho Lee², Jin Woo Lee¹
¹Department of Molecular Medicine, Gachon University College of Medicine, Incheon, Republic of Korea, ²Department of Thoracic and Cardiovascular Surgery, Korea University Anam Hospital, Korea University College of Medicine, Seoul, Republic of Korea, ³Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical Center, Gachon University College of Medicine, Incheon, Republic of Korea
Purpose : Potential of migration of cancer cells is one of the most important factors related with cancer infiltration or metastasis. A cancer migration study in a microgravity environment is an important tool for studying mechanism of metastasis.
Methods : In this study, we evaluated the proliferation and migration of two types (adenocarcinoma A549, squamous cell carcinoma H1703) of non-small cell lung cancers (NSCLC) in a floating environment with microgravity.
Results : When we measured proliferation of two NSCLCs in the microgravity (MG) and ground-gravity (CONT), a normalized proliferation rate of A549 in MG was higher than that in CONT. Wound healing results of A549 and H1703 which related with cancer cell migration potential showed rapid recovery in MG; particularly, the migration rate of A549 was faster than that of H1703 both the normal and low proliferating conditions. Gene expression results showed that the microgravity accelerated the migration of NSCLC. Both A549 and H1703 in MG highly expressed the migration-related genes MMP-2, MMP-9, TIMP-1, and TIMP-2 compared to CONT at 24h. Furthermore, analysis of MMP-2 protein synthesis revealed weaker metastatic performance of H1703 than that of A549.
Conclusion : Therefore, the simulated microgravity affected differently depending on cell type of NSCLC.
Methods : In this study, we evaluated the proliferation and migration of two types (adenocarcinoma A549, squamous cell carcinoma H1703) of non-small cell lung cancers (NSCLC) in a floating environment with microgravity.
Results : When we measured proliferation of two NSCLCs in the microgravity (MG) and ground-gravity (CONT), a normalized proliferation rate of A549 in MG was higher than that in CONT. Wound healing results of A549 and H1703 which related with cancer cell migration potential showed rapid recovery in MG; particularly, the migration rate of A549 was faster than that of H1703 both the normal and low proliferating conditions. Gene expression results showed that the microgravity accelerated the migration of NSCLC. Both A549 and H1703 in MG highly expressed the migration-related genes MMP-2, MMP-9, TIMP-1, and TIMP-2 compared to CONT at 24h. Furthermore, analysis of MMP-2 protein synthesis revealed weaker metastatic performance of H1703 than that of A549.
Conclusion : Therefore, the simulated microgravity affected differently depending on cell type of NSCLC.
첨부파일 : fig1.docx
책임저자: Kuk Hui Son
Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical Center, Gachon University College of Medicine, Incheon, Republic of Korea
발표자: Kuk Hui Son , E-mail : dr632@gilhospital.com