초록접수 현황
| 20F-167 | 구연 발표 |
Modification of anti-thrombotic surface coating for extracorporeal circulatory device
Do-Wan Kim1, Hwa-Jin Cho2, Brad Choi3, In-Seok Jeong1
1. Department of thoracic and cardiovascular surgery, Chonnam National University Hospital and Medical School, Gwangju, Republic of Korea
2. Department of Pediatrics, Chonnam National University Children's Hospital and Medical School, Gwangju, Republic of Korea
3. Noanix, Cheongju-si, Chungcheongbukdo, Republic of Korea
Purpose : Development of novel surface coating modification still remains to be investigated in the extracorporeal circulation technology. We evaluated the anti-thrombotic properties of new developed tube graft with novel surface coating in ex-vivo mock circulation (EVMC) conditions.
Methods : An EVMC system was assembled using a roller pump, polyvinyl chloride (PVC) tube (3/8 inch inner diameter, 50 cm length) and priming solution (human blood 40 ml + normal saline 10 ml). We made 5 tube grafts with novel bilaminar surface coating (inner layer base coating and heparin based hydrophilic outer layer coating). Flow cytometry (CD61b, CD62p) was used to measure the platelet consumption and activity after 2 hours circulation and performed scanning electron microscope (SEM).
Results : After 2 hours of 5 EVMC experiments, there were no thrombus formation inside all PVC tubes. No significant platelet activation and consumption were detected, based on result of flow cytometry study using post-EVMC priming solution. On SEM, almost no platelet aggregation was found on inner surface of PVC tube.
Conclusion : Our novel surface coating method will be promising technology in extracorporeal circulatory device. Further studies are needed to demonstrate the long-term durability.
Methods : An EVMC system was assembled using a roller pump, polyvinyl chloride (PVC) tube (3/8 inch inner diameter, 50 cm length) and priming solution (human blood 40 ml + normal saline 10 ml). We made 5 tube grafts with novel bilaminar surface coating (inner layer base coating and heparin based hydrophilic outer layer coating). Flow cytometry (CD61b, CD62p) was used to measure the platelet consumption and activity after 2 hours circulation and performed scanning electron microscope (SEM).
Results : After 2 hours of 5 EVMC experiments, there were no thrombus formation inside all PVC tubes. No significant platelet activation and consumption were detected, based on result of flow cytometry study using post-EVMC priming solution. On SEM, almost no platelet aggregation was found on inner surface of PVC tube.
Conclusion : Our novel surface coating method will be promising technology in extracorporeal circulatory device. Further studies are needed to demonstrate the long-term durability.
책임저자: In-Seok Jeong
Department of Thoracic and Cardiovascular Surgery, Chonnam National University Hospital and Medical School, Gwangju, Republic of Korea
발표자: Do-Wan Kim, E-mail : maskjoa@naver.com