초록접수 현황
| 16F-046 | 구연 발표 |
Real-Time Computed Tomography Fluoroscopy Guided Solitary Lung Cancer Formation in a Rabbit Model
Hyun Koo Kim¹, Byeong Hyeon Choi¹, Hwan Seok Young², Yu Hua Quan¹, Jiyun Rho¹, Jae Seon Eo³, Kook Nam Han¹, Young Ho Choi¹
¹Department of Thoracic and Cardiovascular Surgery, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea, ²Department of Radiology, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea, ³Department of Nuclear Medicine, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea
Background : Preclinical studies of lung cancer require suitable large animal models to evaluate and develop surgical and interventional techniques. Here, we evaluate the feasibility and safety of a newly developed solitary lung cancer in rabbit model that utilizes real-time computed tomography (CT) fluoroscopy-guided inoculation of VX2 single cell suspensions.
Methods : Thirty-eight rabbits were divided into four groups according to number of VX2 carcinoma cells, lipiodol amount, matrigel amount, and injection needle size. The different VX2 tumor cell suspensions were percutaneously injected into rabbit lungs under real-time CT fluoroscopy guidance. Two weeks later, VX2 lung cancers were confirmed by positron emission tomography/CT, necropsy, and histology.
Results : Real-time CT fluoroscopy allowed the precise inoculation of tumor cell suspensions containing lipiodol. Use of matrigel and a small-sized needle reduced spreading and leakage of tumor cell suspensions in the lung parenchyma. Solitary lung cancers were successfully established in all rabbits in group 4 (22/22, 100%); these rabbits were inoculated with 150 μl VX2 tumor cells filtered through a 100 μm cell strainer, 100 μl lipiodol, and 150 μl matrigel, using 26-gauge needles. This was determined to be the optimal combination. Pneumothorax was observed in only 2 of 38 rabbits (5.3%), and these rabbits survived to the end of the study without any intervention.
Conclusion : Real-time CT fluoroscopy-guided inoculation of the appropriate composition of a VX2 tumor cell suspension using a small sized needle is an easy and safe method to model solitary lung cancer in rabbits.
Methods : Thirty-eight rabbits were divided into four groups according to number of VX2 carcinoma cells, lipiodol amount, matrigel amount, and injection needle size. The different VX2 tumor cell suspensions were percutaneously injected into rabbit lungs under real-time CT fluoroscopy guidance. Two weeks later, VX2 lung cancers were confirmed by positron emission tomography/CT, necropsy, and histology.
Results : Real-time CT fluoroscopy allowed the precise inoculation of tumor cell suspensions containing lipiodol. Use of matrigel and a small-sized needle reduced spreading and leakage of tumor cell suspensions in the lung parenchyma. Solitary lung cancers were successfully established in all rabbits in group 4 (22/22, 100%); these rabbits were inoculated with 150 μl VX2 tumor cells filtered through a 100 μm cell strainer, 100 μl lipiodol, and 150 μl matrigel, using 26-gauge needles. This was determined to be the optimal combination. Pneumothorax was observed in only 2 of 38 rabbits (5.3%), and these rabbits survived to the end of the study without any intervention.
Conclusion : Real-time CT fluoroscopy-guided inoculation of the appropriate composition of a VX2 tumor cell suspension using a small sized needle is an easy and safe method to model solitary lung cancer in rabbits.
책임저자: Hyun Koo Kim
Department of Thoracic and Cardiovascular Surgery, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea
발표자: Hyun Koo Kim, E-mail : kimhyunkoo@korea.ac.kr