초록접수 현황
| 14F-087 | 구연 발표 |
Immunohistochemical Examination of the mTOR Pathway in Stage I Non-small Cell Lung Carcinoma
Su Kyung Hwang¹ Hyeong Ryul Kim¹, Se Jin Jang², Young Soo Park², Duk Hwan Moon¹, Joo Seok¹, Geun Dong Lee¹,Se Hoon Choi¹, Yong-Hee Kim¹, Dong Kwan Kim¹Seung-il Park¹
Department of Thoracic and cardiovascular surgery, Asan Medical Center¹; Departments of Pathology, Asan Medical Center², University of Ulsan College of Medicine, Seoul, South Korea
Background : Previous studies have proven that dysregulation of mammalian target of rapamycin (mTOR) pathway is linked with malignant tumorigenesis. This study explored protein expression profile of mTOR pathway and the relationship with clinicopathologic characteristics in stage I non-small cell lung carcinoma (NSCLC).
Methods : The protein expression profile of PTEN, p-Akt, p-mTOR, p-S6 and eIF4E was examined with tissue microarray method by immunohistochemical staining in 423 patients with stage I NSCLC (250 adenocarcinomas [ADC], 158 squamous cell carcinomas and 15 other carcinomas).
Results : PTEN loss (P < 0.001), p-mTOR (P < 0.001) and p-S6 expression (P = 0.022) were associated with ADC. PTEN loss was correlated with male gender and smoking history while p-mTOR expression was inversely correlated with these factors (P<0.001). In subgroup analysis with ADCs, male gender, smoking history, pT stage, lymphovascular invasion and PTEN loss were poor prognostic factors in univariate analysis. Multivariate analysis indicated that combination of PTEN(-)/p-Akt(+)/p-mTOR(+) showed greater value in determining the prognosis of ADC (hazard ratio = 2.423, P = 0.003) than PTEN alone.
Conclusion : mTOR pathway was activated in early stage of ADCs, suggesting significant role of Akt/mTOR axis in early carcinogenesis. In addition, combination of PTEN(-)/p-Akt(+)/p-mTOR(+) expression was correlated with poor overall survival in lung ADC patients
Methods : The protein expression profile of PTEN, p-Akt, p-mTOR, p-S6 and eIF4E was examined with tissue microarray method by immunohistochemical staining in 423 patients with stage I NSCLC (250 adenocarcinomas [ADC], 158 squamous cell carcinomas and 15 other carcinomas).
Results : PTEN loss (P < 0.001), p-mTOR (P < 0.001) and p-S6 expression (P = 0.022) were associated with ADC. PTEN loss was correlated with male gender and smoking history while p-mTOR expression was inversely correlated with these factors (P<0.001). In subgroup analysis with ADCs, male gender, smoking history, pT stage, lymphovascular invasion and PTEN loss were poor prognostic factors in univariate analysis. Multivariate analysis indicated that combination of PTEN(-)/p-Akt(+)/p-mTOR(+) showed greater value in determining the prognosis of ADC (hazard ratio = 2.423, P = 0.003) than PTEN alone.
Conclusion : mTOR pathway was activated in early stage of ADCs, suggesting significant role of Akt/mTOR axis in early carcinogenesis. In addition, combination of PTEN(-)/p-Akt(+)/p-mTOR(+) expression was correlated with poor overall survival in lung ADC patients
책임저자: Hyeong Ryul Kim
Department of Thoracic and cardiovascular surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
연락처 : Su Kyung Hwang, Tel: 02-3010-3580 , E-mail : victorylina@gmail.com
