초록접수 현황
| 14F-023 | 포스터 발표 |
Hypocrellin B and Paclitaxel-encapsulated Hyaluronic Acid-ceramide Nanoparticles for Lung Cancer Targeted Photodynamic Therapy
Ji-Eun Chang¹, Mi Kyung Bae¹, Sukki Cho¹,², Kwhanmien Kim¹,², and Sanghoon Jheon¹,²
Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, Seongnam-Si, Gyeonggi-do, Republic of Korea¹,
Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine, Seoul, Republic of Korea²
Background : To increase the therapeutic efficacy of photodynamic therapy (PDT) in treating lung cancer, we developed a combination of both photosensitizers and anticancer drugs encapsulated hyaluronic acid-ceramide nanoparticles. Based on one of our previous studies, co-delivery systems of photosensitizers and anticancer agents greatly improve the therapeutic effect of PDT. Furthermore, hyaluronic acid-ceramide-based nanoparticles are ideal targeting carriers for lung cancer.
Methods : In vitro phototoxicity in A549 (human lung carcinoma) cells and in vivo antitumor efficacy in A549 tumor-bearing mice treated with hypocrellin B (HB) loaded nanoparticles (HB-NPs) or hypocrellin B and paclitaxel loaded nanoparticles (HB-P-NPs) were evaluated.
Results : The cell viability assay, microscopic analysis and FACS analysis was performed for the in vitro studies and HB-P-NPs showed enhanced phototoxicity compared with HB-NPs. No significant change in cell viability was observed in the HB-NPs group with 2 J/cm² of PDT, while significant reduction in cell viability was observed in the HB-P-NPs group (p<0.005) under the same PDT conditions (n=3).
In the animal study, the tumor volume change and the histological analysis was studied and the anticancer efficacy improved in the order of free HB < HB-NPs < HB-P-NPs. On day 40, tumor volumes of both HB-P-NPs and HB-NPs treated groups with twice PDT for 200 J/cm² were significantly smaller than free HB treated group (p<0.005 and p<0.05, respectively) (n=4).
Conclusion : In conclusion, the combination therapy of PDT and chemotherapy, and hyaluronic acid-ceramide nanoparticle based targeted delivery improved the effects of PDT in lung cancer.
Methods : In vitro phototoxicity in A549 (human lung carcinoma) cells and in vivo antitumor efficacy in A549 tumor-bearing mice treated with hypocrellin B (HB) loaded nanoparticles (HB-NPs) or hypocrellin B and paclitaxel loaded nanoparticles (HB-P-NPs) were evaluated.
Results : The cell viability assay, microscopic analysis and FACS analysis was performed for the in vitro studies and HB-P-NPs showed enhanced phototoxicity compared with HB-NPs. No significant change in cell viability was observed in the HB-NPs group with 2 J/cm² of PDT, while significant reduction in cell viability was observed in the HB-P-NPs group (p<0.005) under the same PDT conditions (n=3).
In the animal study, the tumor volume change and the histological analysis was studied and the anticancer efficacy improved in the order of free HB < HB-NPs < HB-P-NPs. On day 40, tumor volumes of both HB-P-NPs and HB-NPs treated groups with twice PDT for 200 J/cm² were significantly smaller than free HB treated group (p<0.005 and p<0.05, respectively) (n=4).
Conclusion : In conclusion, the combination therapy of PDT and chemotherapy, and hyaluronic acid-ceramide nanoparticle based targeted delivery improved the effects of PDT in lung cancer.
책임저자: 전상훈
서울대학교 의과대학 흉부외과학교실
연락처 : 장지은, Tel: 031-787-8109 , E-mail : 97048@snubh.org
