초록접수 현황
| 14F-150 | 포럼 발표 |
Calcification of Decellularized and Alpha-galactosidase Treated Bovine Pericardial Tissue in an Alpha-gal Knock Out Mouse Implantation Model: Comparison with Primate Pericardial Tissue
김민석, 임홍국, 김용진
서울대학교 의과대학 서울대학교병원 흉부외과학교실
Background : The aims of this study were (1)to compare calcification and immune reactions of glutaraldehyde(GA)-fixed bovine with primate pericardium and (2)to assess the differences in bovine pericardium treated with decellularization and α-galactosidase, using α-Gal(Galα1,3-Galβ1-4GlcNAc-R) knock-out(KO) mouse implantation model.
Methods : Four types of GA-fixed xenogenic tissues were implanted into α-Gal KO mice(Group 1: primate pericardium; Group 2: bovine pericardium; Group 3: decellularized bovine pericardium; Group 4: decellularized and α-galactosidase treated bovine pericardium). Serum enzyme-linked immunosorbent assays for immune response were performed before and after the implantation. Implanted tissues were harvested and studied for histopathology and quantification of calcification.
Results : Anti-α-Gal IgG antibody titers of Groups 2-4 increased after implantation although the titers did not change in Group 1. When compared with pre-implantation titers, the increased IgG titers were highest at 8 weeks after implantation in Groups 2-4 (0.4358±0.0524 vs 0.5462±0.0519 in Group 2, 0.4712±0.0500 vs 0.6424±0.0613 in Group 3, and 0.4892±0.0801 vs 0.6112±0.0339 in Group 4; p=0.028 in each group). Calcium levels were higher in Group 2 than in Group 1 (10.85±4.30 vs 4.80±2.34㎍/mg, p<0.001), but were not significantly higher in Groups 3 and 4 than in Group 1 (8.02±7.80㎍/mg in Group 3, p=0.904; 6.80±5.38㎍/mg in Group 4, p=0.210).
Conclusion : GA-fixed bovine pericardium showed higher calcium levels than primate pericardium. Increased IgG antibody titers in the bovine but not in the primate group suggested that immune response might be involved in implanted tissue. Differences in calcium levels of bovine pericardium treated with decellularization and α-galactosidase were insignificant compared with primate pericardium.
Methods : Four types of GA-fixed xenogenic tissues were implanted into α-Gal KO mice(Group 1: primate pericardium; Group 2: bovine pericardium; Group 3: decellularized bovine pericardium; Group 4: decellularized and α-galactosidase treated bovine pericardium). Serum enzyme-linked immunosorbent assays for immune response were performed before and after the implantation. Implanted tissues were harvested and studied for histopathology and quantification of calcification.
Results : Anti-α-Gal IgG antibody titers of Groups 2-4 increased after implantation although the titers did not change in Group 1. When compared with pre-implantation titers, the increased IgG titers were highest at 8 weeks after implantation in Groups 2-4 (0.4358±0.0524 vs 0.5462±0.0519 in Group 2, 0.4712±0.0500 vs 0.6424±0.0613 in Group 3, and 0.4892±0.0801 vs 0.6112±0.0339 in Group 4; p=0.028 in each group). Calcium levels were higher in Group 2 than in Group 1 (10.85±4.30 vs 4.80±2.34㎍/mg, p<0.001), but were not significantly higher in Groups 3 and 4 than in Group 1 (8.02±7.80㎍/mg in Group 3, p=0.904; 6.80±5.38㎍/mg in Group 4, p=0.210).
Conclusion : GA-fixed bovine pericardium showed higher calcium levels than primate pericardium. Increased IgG antibody titers in the bovine but not in the primate group suggested that immune response might be involved in implanted tissue. Differences in calcium levels of bovine pericardium treated with decellularization and α-galactosidase were insignificant compared with primate pericardium.
책임저자: 김용진
서울대학교 의과대학 서울대학교병원 흉부외과학교실
연락처 : 김민석, Tel: 02-732-3285 , E-mail : mesmerist84@gmail.com
